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SlimR: Machine Learning-Assisted, Marker-Based Tool for Single-Cell and Spatial Transcriptomics Annotation

Annotates single-cell and spatial-transcriptomic (ST) data using marker datasets. Supports unified markers list ('Markers_list') creation from built-in databases (e.g., 'Cellmarker2', 'PanglaoDB', 'scIBD', 'TCellSI'), Seurat objects, or user-supplied Excel files. SlimR can predict calculate parameters by machine learning algorithms (e.g., 'Random Forest', 'Gradient Boosting', 'Support Vector Machine', 'Ensemble Learning'), and based on Markers_list, calculate gene expression of different cell types and predict annotation information and calculate corresponding AUC and annotate it, then verify it. At the same time, it can calculate gene expression corresponding to the cell type to generate a reference map for manual annotation (e.g., 'Heat Map', 'Feature Plots', 'Combined Plots'). For more details see Kabacoff (2020, ISBN:9787115420572).

Version: 1.0.8
Depends: R (≥ 3.5)
Imports: cowplot, dplyr, ggplot2, patchwork, pheatmap, readxl, scales, Seurat, tidyr, tools, tibble
Suggests: crayon, caret, gbm, lattice
Published: 2025-10-08
DOI: 10.32614/CRAN.package.SlimR
Author: Zhaoqing Wang ORCID iD [aut, cre]
Maintainer: Zhaoqing Wang <zhaoqingwang at mail.sdu.edu.cn>
BugReports: https://github.com/Zhaoqing-wang/SlimR/issues
License: MIT + file LICENSE
URL: https://github.com/Zhaoqing-wang/SlimR
NeedsCompilation: no
Materials: README, NEWS
CRAN checks: SlimR results

Documentation:

Reference manual: SlimR.html , SlimR.pdf

Downloads:

Package source: SlimR_1.0.8.tar.gz
Windows binaries: r-devel: SlimR_1.0.8.zip, r-release: SlimR_1.0.7.zip, r-oldrel: SlimR_1.0.7.zip
macOS binaries: r-release (arm64): SlimR_1.0.8.tgz, r-oldrel (arm64): SlimR_1.0.8.tgz, r-release (x86_64): SlimR_1.0.8.tgz, r-oldrel (x86_64): SlimR_1.0.8.tgz
Old sources: SlimR archive

Linking:

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These binaries (installable software) and packages are in development.
They may not be fully stable and should be used with caution. We make no claims about them.
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