The hardware and bandwidth for this mirror is donated by dogado GmbH, the Webhosting and Full Service-Cloud Provider. Check out our Wordpress Tutorial.
If you wish to report a bug, or if you are interested in having us mirror your free-software or open-source project, please feel free to contact us at mirror[@]dogado.de.

Quickstart Tutorial

In this tutorial, we will construct a Symphony reference from two PBMC datasets from 2 technologies (10x 3’v1 and 3’v2), then map a third dataset from a new technology (10x 5’) with Symphony. The analysis follows from Fig. 2 of the paper (but with downsampled datasets to fit within CRAN limits on subdirectory size).

Installation

Install Symphony with standard commands.

install.packages('symphony')

Once Symphony is installed, load it up!

library(symphony)

# Other packages for this tutorial
suppressPackageStartupMessages({
    # Analysis
    library(harmony)
    library(irlba)
    library(data.table)
    library(dplyr)

    # Plotting
    library(ggplot2)
    library(ggthemes)
    library(ggrastr)
    library(RColorBrewer)
})

Load the data

Get the expression and metadata.

load('../data/pbmcs_exprs_small.rda')
load('../data/pbmcs_meta_small.rda')

dim(pbmcs_exprs_small)

## [1] 1767 1200

dim(pbmcs_meta_small)

## [1] 1200    7

pbmcs_meta_small %>% head(4)

##                            cell_id donor nUMI nGene percent_mito cell_type
## 17642   fivePrime_GATCGCGAGACCGGAT    5p 6580  2020   0.05714286         B
## 4820  threepfresh_GATCGTACAAAGGTGC  3pv2 4426  1207   0.03366471     T_CD8
## 13347   fivePrime_AACTCAGGTTACGCGC    5p 4750  1670   0.05642105         B
## 10634      threepv1_CGTGATGAGTCCTC  3pv1 4121  1228   0.01504489     T_CD4
##       cell_type_broad
## 17642               B
## 4820                T
## 13347               B
## 10634               T

Subset the dataset into reference and query.

idx_query = which(pbmcs_meta_small$donor == "5p") # use 5' dataset as the query
ref_exp_full = pbmcs_exprs_small[, -idx_query]
ref_metadata = pbmcs_meta_small[-idx_query, ]
query_exp = pbmcs_exprs_small[, idx_query]
query_metadata = pbmcs_meta_small[idx_query, ]

Build Symphony reference

There are two options for how to build a Symphony reference. Option 1 (buildReferenceFromHarmonyObj) is the more modular option, meaning that the user has more control over the preprocessing steps prior to reference compression. Option 2 (buildReference) builds a reference starting from expression, automating the procedure more but offering less flexibility.

We’ll demonstrate both options below.

Option 1: Build from Harmony object (preferred method)

This option consists of more steps than Option 2 but allows your code to be more modular and flexible if you want to do your own preprocessing steps before the Harmony integration step. We recommend this option for most users.

It is important to generate vargenes_means_sds (containing variable gene means and standard deviations used to scale the genes) as well as save the loadings for the PCA step.

Starting with the reference expression,

ref_exp_full[1:5, 1:2] # Sparse matrix with the normalized genes x cells matrix

## 5 x 2 sparse Matrix of class "dgCMatrix"
##         threepfresh_GATCGTACAAAGGTGC threepv1_CGTGATGAGTCCTC
## LYZ                         1.181536                1.766987
## FTL                         2.678021                2.370840
## HLA-DRA                     1.708152                .       
## CD74                        1.181536                1.766987
## S100A4                      1.708152                2.113817

Select variable genes and subset reference expression by variable genes (the command below will select the top 1,000 genes per batch, then pool them)

var_genes = vargenes_vst(ref_exp_full, groups = as.character(ref_metadata[['donor']]), topn = 1000)
ref_exp = ref_exp_full[var_genes, ]
dim(ref_exp)

## [1] 1600  770

Calculate and save the mean and standard deviations for each gene

vargenes_means_sds = tibble(symbol = var_genes, mean = Matrix::rowMeans(ref_exp))
vargenes_means_sds$stddev = rowSDs(ref_exp, vargenes_means_sds$mean)
head(vargenes_means_sds)

## # A tibble: 6 × 3
##   symbol   mean stddev
##   <chr>   <dbl>  <dbl>
## 1 LYZ      1.78   1.91
## 2 HLA-DRA  1.87   1.69
## 3 FTL      3.55   1.22
## 4 CD74     2.54   1.53
## 5 S100A9   1.62   1.83
## 6 S100A4   2.53   1.46

Scale data using calculated gene means and standard deviations

ref_exp_scaled = scaleDataWithStats(ref_exp, vargenes_means_sds$mean, vargenes_means_sds$stddev, 1)

Run PCA (using SVD), save gene loadings (s$u)

set.seed(0)
s = irlba(ref_exp_scaled, nv = 20)
Z_pca_ref = diag(s$d) %*% t(s$v) # [pcs by cells]
loadings = s$u

Run Harmony integration. It is important to set return_object = TRUE.

set.seed(0)
ref_harmObj = harmony::HarmonyMatrix(
        data_mat = t(Z_pca_ref),  ## PCA embedding matrix of cells
        meta_data = ref_metadata, ## dataframe with cell labels
        theta = c(2),             ## cluster diversity enforcement
        vars_use = c('donor'),    ## variable to integrate out
        nclust = 100,             ## number of clusters in Harmony model
        max.iter.harmony = 20,    ## max number of iterations
        return_object = TRUE,     ## return the full Harmony model object
        do_pca = FALSE            ## don't recompute PCs
)

## Harmony 1/20

## Harmony 2/20

## Harmony 3/20

## Harmony 4/20

## Harmony 5/20

## Harmony 6/20

## Harmony 7/20

## Harmony 8/20

## Harmony 9/20

## Harmony converged after 9 iterations

To run the next function buildReferenceFromHarmonyObj(), you need to input the saved gene loadings (loadings) and vargenes_means_sds.

# Compress a Harmony object into a Symphony reference
reference = buildReferenceFromHarmonyObj(
                        ref_harmObj,            # output object from HarmonyMatrix()
                        ref_metadata,           # reference cell metadata
                        vargenes_means_sds,     # gene names, means, and std devs for scaling
                        loadings,               # genes x PCs matrix
                        verbose = TRUE,         # verbose output
                        do_umap = TRUE,         # set to TRUE to run UMAP
                        save_uwot_path = './testing_uwot_model_1') # file path to save uwot model

## Save metadata, vargenes (S), and loadings (U)

## Save R, Z_orig, Z_corr, and betas from Harmony object

## Calculate final L2 normalized reference centroids (Y_cos)

## Calculate reference compression terms (Nr and C)

## UMAP

## File already exists at that path... overwriting...

## Warning: invalid uid value replaced by that for user 'nobody'

## Saved uwot model

## Finished nicely.

Save Symphony reference for later mapping (modify with your desired output path)

saveRDS(reference, './testing_reference1.rds')

Let’s take a look at what the reference object contains: * meta_data: metadata * vargenes: variable genes, means, and standard deviations used for scaling * loadings: gene loadings for projection into pre-Harmony PC space * R: Soft cluster assignments * Z_orig: Pre-Harmony PC embedding * Z_corr: Harmonized PC embedding * centroids: locations of final Harmony soft cluster centroids * cache: pre-calculated reference-dependent portions of the mixture model * umap: UMAP coordinates * save_uwot_path: path to saved uwot model (for query UMAP projection into reference UMAP coordinates)

str(reference)

## List of 12
##  $ meta_data     :'data.frame':  770 obs. of  7 variables:
##   ..$ cell_id        : chr [1:770] "threepfresh_GATCGTACAAAGGTGC" "threepv1_CGTGATGAGTCCTC" "threepv1_AAGAATCTTGGAGG" "threepfresh_CTGAAACCACACTGCG" ...
##   ..$ donor          : chr [1:770] "3pv2" "3pv1" "3pv1" "3pv2" ...
##   ..$ nUMI           : int [1:770] 4426 4121 1535 11956 6273 1840 2702 4260 3436 3966 ...
##   ..$ nGene          : int [1:770] 1207 1228 560 2685 1615 584 945 1188 1360 1486 ...
##   ..$ percent_mito   : num [1:770] 0.0337 0.015 0.0261 0.0278 0.0289 ...
##   ..$ cell_type      : chr [1:770] "T_CD8" "T_CD4" "B" "DC" ...
##   ..$ cell_type_broad: chr [1:770] "T" "T" "B" "DC" ...
##  $ vargenes      : tibble [1,600 × 3] (S3: tbl_df/tbl/data.frame)
##   ..$ symbol: chr [1:1600] "LYZ" "HLA-DRA" "FTL" "CD74" ...
##   ..$ mean  : Named num [1:1600] 1.78 1.87 3.55 2.54 1.62 ...
##   .. ..- attr(*, "names")= chr [1:1600] "LYZ" "HLA-DRA" "FTL" "CD74" ...
##   ..$ stddev: Named num [1:1600] 1.91 1.69 1.22 1.53 1.83 ...
##   .. ..- attr(*, "names")= chr [1:1600] "LYZ" "HLA-DRA" "FTL" "CD74" ...
##  $ loadings      : num [1:1600, 1:20] 0.1247 0.0811 0.1181 0.0621 0.118 ...
##  $ R             : num [1:100, 1:770] 7.10e-11 3.34e-02 2.47e-06 3.32e-02 5.24e-10 ...
##  $ Z_orig        : num [1:20, 1:770] -4.332 -0.976 3.186 0.138 -0.154 ...
##   ..- attr(*, "dimnames")=List of 2
##   .. ..$ : chr [1:20] "PC_1" "PC_2" "PC_3" "PC_4" ...
##   .. ..$ : chr [1:770] "4820" "10634" "8538" "4091" ...
##  $ Z_corr        : num [1:20, 1:770] -4.334 -0.977 3.521 -0.833 0.222 ...
##   ..- attr(*, "dimnames")=List of 2
##   .. ..$ : chr [1:20] "harmony_1" "harmony_2" "harmony_3" "harmony_4" ...
##   .. ..$ : chr [1:770] "4820" "10634" "8538" "4091" ...
##  $ betas         : num [1:3, 1:20, 1:100] -4.681788 0.011596 -0.011596 -6.017041 0.000601 ...
##  $ centroids     : num [1:20, 1:100] -0.21171 -0.2733 -0.36088 0.10265 0.00121 ...
##  $ cache         :List of 2
##   ..$ : num [1:100, 1] 1.91 7.81 8.21 7.81 5.64 ...
##   ..$ : num [1:100, 1:20] -8.9 -37.9 -34.6 -38 -21.2 ...
##  $ centroids_pc  :'data.frame':  100 obs. of  20 variables:
##   ..$ harmony_1 : num [1:100] -4.66 -4.86 -4.21 -4.86 -3.75 ...
##   ..$ harmony_2 : num [1:100] -6.019 -0.792 -3.614 -0.793 11.907 ...
##   ..$ harmony_3 : num [1:100] -7.949 3.73 0.606 3.729 -4.487 ...
##   ..$ harmony_4 : num [1:100] 2.261 -0.331 0.449 -0.33 -0.267 ...
##   ..$ harmony_5 : num [1:100] 0.0266 0.1799 -0.3958 0.1795 1.2473 ...
##   ..$ harmony_6 : num [1:100] 1.995 -0.295 0.933 -0.295 -0.557 ...
##   ..$ harmony_7 : num [1:100] 0.878 0.887 -1.859 0.889 0.662 ...
##   ..$ harmony_8 : num [1:100] 1.692 0.858 -2.703 0.861 -0.441 ...
##   ..$ harmony_9 : num [1:100] -3.4883 -0.0192 1.9045 -0.0262 -1.9898 ...
##   ..$ harmony_10: num [1:100] -4.18151 0.07435 -0.14573 0.07504 -0.00894 ...
##   ..$ harmony_11: num [1:100] -2.1811 0.0718 -1.7295 0.0733 -0.5961 ...
##   ..$ harmony_12: num [1:100] -3.3362 0.0511 0.4145 0.0513 1.5912 ...
##   ..$ harmony_13: num [1:100] 2.348 0.338 -0.489 0.337 0.132 ...
##   ..$ harmony_14: num [1:100] -6.7619 -0.0479 -0.4441 -0.0482 4.0588 ...
##   ..$ harmony_15: num [1:100] 7.69 -0.241 0.328 -0.245 0.437 ...
##   ..$ harmony_16: num [1:100] 0.9247 0.0268 0.7702 0.025 -1.2835 ...
##   ..$ harmony_17: num [1:100] -3.9477 -0.0965 -0.6243 -0.097 1.0399 ...
##   ..$ harmony_18: num [1:100] 9.9037 -0.0592 0.8881 -0.0584 -3.5468 ...
##   ..$ harmony_19: num [1:100] 6.7289 -0.0746 -0.6049 -0.0739 2.7701 ...
##   ..$ harmony_20: num [1:100] -5.959 -0.319 0.62 -0.323 -4.712 ...
##  $ umap          :List of 1
##   ..$ embedding: num [1:770, 1:2] -10.71 -9.44 12.07 12.85 11.76 ...
##   .. ..- attr(*, "scaled:center")= num [1:2] -0.679 -2.649
##   .. ..- attr(*, "dimnames")=List of 2
##   .. .. ..$ : chr [1:770] "4820" "10634" "8538" "4091" ...
##   .. .. ..$ : chr [1:2] "UMAP1" "UMAP2"
##  $ save_uwot_path: chr "./testing_uwot_model_1"

The harmonized embedding is located in the Z_corr slot of the reference object.

dim(reference$Z_corr)

## [1]  20 770

reference$Z_corr[1:5, 1:5]

##                 4820      10634       8538       4091       11682
## harmony_1 -4.3340226 -4.2434240 -4.4843546  8.9367094 12.47204697
## harmony_2 -0.9768383 -1.2437083 13.2156410  2.4408398  0.09292606
## harmony_3  3.5205608  3.2172170 -4.6302237 -0.2076788 -0.29597939
## harmony_4 -0.8331292 -0.5120756  0.2940588 -0.4150630 -4.93994465
## harmony_5  0.2217745 -0.8703953  1.4084306 -3.8374885 -0.03576764

Visualize reference UMAP

reference = readRDS('./testing_reference1.rds')
umap_labels = cbind(ref_metadata, reference$umap$embedding)
plotBasic(umap_labels, title = 'Reference', color.mapping = pbmc_colors)

Option 2: Build from scratch (starting with expression)

This option computes a reference object starting from expression in a unified pipeline, automating the preprocessing steps.

# Build reference
set.seed(0)
reference = symphony::buildReference(
            ref_exp_full,
            ref_metadata,
            vars = c('donor'),         # variables to integrate over
            K = 100,                   # number of Harmony clusters
            verbose = TRUE,            # verbose output
            do_umap = TRUE,            # can set to FALSE if want to run umap separately later
            do_normalize = FALSE,      # set to TRUE if input counts are not normalized yet
            vargenes_method = 'vst',   # method for variable gene selection ('vst' or 'mvp')
            vargenes_groups = 'donor', # metadata column specifying groups for variable gene selection 
            topn = 1000,               # number of variable genes to choose per group
            d = 20,                    # number of PCs
            save_uwot_path = './testing_uwot_model_2' # file path to save uwot model
)

## Finding variable genes using vst method

## Total 1600 genes for downstream steps

## Scaling and PCA

## Running Harmony integration

## Harmony 1/20

## Harmony 2/20

## Harmony 3/20

## Harmony 4/20

## Harmony 5/20

## Harmony 6/20

## Harmony 7/20

## Harmony 8/20

## Harmony 9/20

## Harmony 10/20

## Harmony converged after 10 iterations

## Computing reference compression terms

## Running UMAP

## File already exists at that path... overwriting...

## Warning: invalid uid value replaced by that for user 'nobody'

## Saved uwot model

# Save reference (modify with your desired output path)
saveRDS(reference, './testing_reference2.rds')

Visualize reference UMAP

reference = readRDS('./testing_reference2.rds')
umap_labels = cbind(ref_metadata, reference$umap$embedding)
plotBasic(umap_labels, title = 'Reference', color.mapping = pbmc_colors)

Map query

In order to map a new query dataset onto the reference, you will need a reference object saved from the steps above, as well as query cell expression and metadata.

The query dataset is assumed to have been normalized in the same manner as the reference cells (here, default is log(CP10k+1) normalization).

# Read in Symphony reference to map to
reference = readRDS('./testing_reference1.rds')
# Map query
query = mapQuery(query_exp,             # query gene expression (genes x cells)
                 query_metadata,        # query metadata (cells x attributes)
                 reference,             # Symphony reference object
                 do_normalize = FALSE,  # perform log(CP10k) normalization on query
                 do_umap = TRUE)        # project query cells into reference UMAP

## Scaling and synchronizing query gene expression

## Found 1600 out of 1600 reference variable genes in query dataset

## Project query cells using reference gene loadings

## Clustering query cells to reference centroids

## Correcting query batch effects

## UMAP

## All done!

Note: Symphony assumes that the query is normalized in the same manner as the reference. Our default implementation currently uses log(CP10k+1) normalization.

Let’s take a look at what the query object contains: * Z: query cells in reference Harmonized embedding * Zq_pca: query cells in pre-Harmony reference PC embedding (prior to correction) * R: query cell soft cluster assignments * Xq: query cell design matrix for correction step * umap: query cells projected into reference UMAP coordinates (using uwot) * meta_data: metadata

str(query)

## List of 7
##  $ exp      :Formal class 'dgCMatrix' [package "Matrix"] with 6 slots
##   .. ..@ i       : int [1:99120] 1 2 3 5 10 13 14 15 26 27 ...
##   .. ..@ p       : int [1:431] 0 267 497 727 942 1197 1384 1608 1877 2047 ...
##   .. ..@ Dim     : int [1:2] 1767 430
##   .. ..@ Dimnames:List of 2
##   .. .. ..$ : chr [1:1767] "LYZ" "FTL" "HLA-DRA" "CD74" ...
##   .. .. ..$ : chr [1:430] "fivePrime_GATCGCGAGACCGGAT" "fivePrime_AACTCAGGTTACGCGC" "fivePrime_ACATCAGGTCTTGTCC" "fivePrime_GCCTCTATCATGTCCC" ...
##   .. ..@ x       : num [1:99120] 2.15 3.9 5.41 3.4 3.17 ...
##   .. ..@ factors : list()
##  $ meta_data:'data.frame':   430 obs. of  7 variables:
##   ..$ cell_id        : chr [1:430] "fivePrime_GATCGCGAGACCGGAT" "fivePrime_AACTCAGGTTACGCGC" "fivePrime_ACATCAGGTCTTGTCC" "fivePrime_GCCTCTATCATGTCCC" ...
##   ..$ donor          : chr [1:430] "5p" "5p" "5p" "5p" ...
##   ..$ nUMI           : int [1:430] 6580 4750 4432 5831 5558 5360 4749 5152 4929 4194 ...
##   ..$ nGene          : int [1:430] 2020 1670 1668 2027 1890 1388 1661 1805 1417 1575 ...
##   ..$ percent_mito   : num [1:430] 0.0571 0.0564 0.0399 0.0326 0.0587 ...
##   ..$ cell_type      : chr [1:430] "B" "B" "B" "T_CD4" ...
##   ..$ cell_type_broad: chr [1:430] "B" "B" "B" "T" ...
##  $ Z        : num [1:20, 1:430] -3.375 13.148 -5.845 -0.137 0.288 ...
##   ..- attr(*, "dimnames")=List of 2
##   .. ..$ : chr [1:20] "harmony_1" "harmony_2" "harmony_3" "harmony_4" ...
##   .. ..$ : chr [1:430] "17642" "13347" "13638" "17934" ...
##  $ Zq_pca   : num [1:20, 1:430] -3.089 14.01 -6.805 1.207 -0.721 ...
##   ..- attr(*, "dimnames")=List of 2
##   .. ..$ : chr [1:20] "PC_1" "PC_2" "PC_3" "PC_4" ...
##   .. ..$ : chr [1:430] "17642" "13347" "13638" "17934" ...
##  $ R        : num [1:100, 1:430] 2.90e-09 2.79e-11 3.66e-11 2.79e-11 1.67e-03 ...
##  $ Xq       :Formal class 'dgCMatrix' [package "Matrix"] with 6 slots
##   .. ..@ i       : int [1:860] 0 1 0 1 0 1 0 1 0 1 ...
##   .. ..@ p       : int [1:431] 0 2 4 6 8 10 12 14 16 18 ...
##   .. ..@ Dim     : int [1:2] 2 430
##   .. ..@ Dimnames:List of 2
##   .. .. ..$ : NULL
##   .. .. ..$ : NULL
##   .. ..@ x       : num [1:860] 1 1 1 1 1 1 1 1 1 1 ...
##   .. ..@ factors : list()
##  $ umap     : num [1:430, 1:2] 11.55 11.35 11.39 -8.22 11.93 ...
##   ..- attr(*, "dimnames")=List of 2
##   .. ..$ : chr [1:430] "17642" "13347" "13638" "17934" ...
##   .. ..$ : chr [1:2] "UMAP1" "UMAP2"

Predict query cell types using k-NN. Setting confidence = TRUE also returns the prediction confidence scores (proportion of neighbors with winning vote).

query = knnPredict(query,       # query object
                   reference,   # reference object
                   reference$meta_data$cell_type, # reference cell labels for training
                   k = 5,       # number of reference neighbors to use for prediction
                   confidence = TRUE)

Query cell type predictions are now in the cell_type_pred_knn column.

head(query$meta_data)

##                          cell_id donor nUMI nGene percent_mito cell_type
## 17642 fivePrime_GATCGCGAGACCGGAT    5p 6580  2020   0.05714286         B
## 13347 fivePrime_AACTCAGGTTACGCGC    5p 4750  1670   0.05642105         B
## 13638 fivePrime_ACATCAGGTCTTGTCC    5p 4432  1668   0.03993682         B
## 17934 fivePrime_GCCTCTATCATGTCCC    5p 5831  2027   0.03258446     T_CD4
## 14432 fivePrime_AGGGATGGTGGGTATG    5p 5558  1890   0.05865419         B
## 18442 fivePrime_GGGACCTCATGCCACG    5p 5360  1388   0.03750000     T_CD4
##       cell_type_broad cell_type_pred_knn cell_type_pred_knn_prob
## 17642               B                  B                     1.0
## 13347               B                  B                     1.0
## 13638               B                  B                     1.0
## 17934               T              T_CD4                     1.0
## 14432               B                  B                     1.0
## 18442               T              T_CD4                     0.8

Visualization of mapping

# Sync the column names for both data frames
reference$meta_data$cell_type_pred_knn = NA
reference$meta_data$cell_type_pred_knn_prob = NA
reference$meta_data$ref_query = 'reference'
query$meta_data$ref_query = 'query'

# Add the UMAP coordinates to the metadata
meta_data_combined = rbind(query$meta_data, reference$meta_data)
umap_combined = rbind(query$umap, reference$umap$embedding)
umap_combined_labels = cbind(meta_data_combined, umap_combined)

# Plot UMAP visualization of all cells
plotBasic(umap_combined_labels, title = 'Reference and query cells', color.by = 'ref_query')

Plot the reference and query side by side.

plotBasic(umap_combined_labels, title = 'Reference and query cells', 
          color.mapping = pbmc_colors, facet.by = 'ref_query')

And that’s a wrap! If you run into issues or have questions about Symphony or this tutorial, please open an issue on GitHub.

sessionInfo()

## R version 4.2.2 (2022-10-31)
## Platform: x86_64-conda-linux-gnu (64-bit)
## Running under: Red Hat Enterprise Linux Server release 6.5 (Santiago)
## 
## Matrix products: default
## BLAS/LAPACK: /PHShome/jbk37/anaconda3/envs/R_v4.2.2/lib/libopenblasp-r0.3.21.so
## 
## locale:
##  [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
##  [3] LC_TIME=en_US.UTF-8        LC_COLLATE=C              
##  [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
##  [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
##  [9] LC_ADDRESS=C               LC_TELEPHONE=C            
## [11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       
## 
## attached base packages:
## [1] stats     graphics  grDevices utils     datasets  methods   base     
## 
## other attached packages:
##  [1] RColorBrewer_1.1-3 ggrastr_1.0.1      ggthemes_4.2.4     ggplot2_3.4.0     
##  [5] dplyr_1.0.10       data.table_1.14.6  irlba_2.3.5.1      Matrix_1.5-3      
##  [9] harmony_0.1.1      Rcpp_1.0.9         symphony_0.1.1    
## 
## loaded via a namespace (and not attached):
##  [1] beeswarm_0.4.0   tidyselect_1.2.0 xfun_0.36        bslib_0.4.2     
##  [5] purrr_1.0.1      lattice_0.20-45  colorspace_2.0-3 vctrs_0.5.1     
##  [9] generics_0.1.3   htmltools_0.5.4  yaml_2.3.6       utf8_1.2.2      
## [13] rlang_1.0.6      jquerylib_0.1.4  pillar_1.8.1     glue_1.6.2      
## [17] withr_2.5.0      uwot_0.1.14      lifecycle_1.0.3  stringr_1.5.0   
## [21] munsell_0.5.0    gtable_0.3.1     codetools_0.2-18 evaluate_0.19   
## [25] labeling_0.4.2   knitr_1.41       fastmap_1.1.0    Cairo_1.6-0     
## [29] vipor_0.4.5      class_7.3-20     fansi_1.0.3      highr_0.10      
## [33] scales_1.2.1     cachem_1.0.6     jsonlite_1.8.4   farver_2.1.1    
## [37] RANN_2.6.1       digest_0.6.31    stringi_1.7.12   grid_4.2.2      
## [41] cowplot_1.1.1    cli_3.6.0        tools_4.2.2      magrittr_2.0.3  
## [45] RcppAnnoy_0.0.20 sass_0.4.4       tibble_3.1.8     pkgconfig_2.0.3 
## [49] ggbeeswarm_0.7.1 rmarkdown_2.19   R6_2.5.1         compiler_4.2.2

These binaries (installable software) and packages are in development.
They may not be fully stable and should be used with caution. We make no claims about them.
Health stats visible at Monitor.