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scimo provides extra recipes steps for dealing with omics data, while also being adaptable to other data types.
You can install scimo from GitHub with:
The cheese_abundance dataset describes fungal community abundance of 74 Amplicon Sequences Variants (ASVs) sampled from the surface of three different French cheeses.
library(scimo)
data("cheese_abundance", "cheese_taxonomy")
cheese_abundance
#> # A tibble: 9 × 77
#> sample cheese rind_type asv_01 asv_02 asv_03 asv_04 asv_05 asv_06 asv_07 asv_08 asv_09 asv_10 asv_11 asv_12 asv_13 asv_14 asv_15 asv_16
#> <chr> <chr> <chr> <dbl> <dbl> <dbl> <dbl> <dbl> <dbl> <dbl> <dbl> <dbl> <dbl> <dbl> <dbl> <dbl> <dbl> <dbl> <dbl>
#> 1 sample1-1 Saint-Ne… Natural 1 0 38 40 1 2 31 8 15 20076 160 92 64 24 51 0
#> 2 sample1-2 Saint-Ne… Natural 3 4 38 61 4 4 48 14 20 32101 403 143 165 39 104 1
#> 3 sample1-3 Saint-Ne… Natural 28 16 33 23 31 29 21 1 7 12921 134 53 55 16 45 2
#> 4 sample2-1 Livarot Washed 0 2 1 0 5 1 0 0 0 7823 2 0 0 42 0 2
#> 5 sample2-2 Livarot Washed 0 0 4 0 1 1 2 0 0 6740 4 1 0 45 0 1
#> 6 sample2-3 Livarot Washed 0 1 2 0 2 1 0 0 0 7484 6 1 0 43 0 7
#> 7 sample3-1 Epoisses Washed 4 2 3 0 2 5 0 0 0 2486 1 1 1 23 0 24
#> 8 sample3-2 Epoisses Washed 0 0 0 0 0 0 0 0 0 3686 2 0 0 28 0 54
#> 9 sample3-3 Epoisses Washed 0 0 1 0 0 0 2 0 0 2988 2 1 0 22 0 36
#> # ℹ 58 more variables: asv_17 <dbl>, asv_18 <dbl>, asv_19 <dbl>, asv_20 <dbl>, asv_21 <dbl>, asv_22 <dbl>, asv_23 <dbl>, asv_24 <dbl>,
#> # asv_25 <dbl>, asv_26 <dbl>, asv_27 <dbl>, asv_28 <dbl>, asv_29 <dbl>, asv_30 <dbl>, asv_31 <dbl>, asv_32 <dbl>, asv_33 <dbl>,
#> # asv_34 <dbl>, asv_35 <dbl>, asv_36 <dbl>, asv_37 <dbl>, asv_38 <dbl>, asv_39 <dbl>, asv_40 <dbl>, asv_41 <dbl>, asv_42 <dbl>,
#> # asv_43 <dbl>, asv_44 <dbl>, asv_45 <dbl>, asv_46 <dbl>, asv_47 <dbl>, asv_48 <dbl>, asv_49 <dbl>, asv_50 <dbl>, asv_51 <dbl>,
#> # asv_52 <dbl>, asv_53 <dbl>, asv_54 <dbl>, asv_55 <dbl>, asv_56 <dbl>, asv_57 <dbl>, asv_58 <dbl>, asv_59 <dbl>, asv_60 <dbl>,
#> # asv_61 <dbl>, asv_62 <dbl>, asv_63 <dbl>, asv_64 <dbl>, asv_65 <dbl>, asv_66 <dbl>, asv_67 <dbl>, asv_68 <dbl>, asv_69 <dbl>,
#> # asv_70 <dbl>, asv_71 <dbl>, asv_72 <dbl>, asv_73 <dbl>, asv_74 <dbl>
glimpse(cheese_taxonomy)
#> Rows: 74
#> Columns: 9
#> $ asv <chr> "asv_01", "asv_02", "asv_03", "asv_04", "asv_05", "asv_06", "asv_07", "asv_08", "asv_09", "asv_10", "asv_11", "asv_12", "asv_…
#> $ lineage <chr> "k__Fungi|p__Ascomycota|c__Dothideomycetes|o__Dothideales|f__Dothioraceae|g__Aureobasidium|s__Aureobasidium_Group_pullulans",…
#> $ kingdom <chr> "Fungi", "Fungi", "Fungi", "Fungi", "Fungi", "Fungi", "Fungi", "Fungi", "Fungi", "Fungi", "Fungi", "Fungi", "Fungi", "Fungi",…
#> $ phylum <chr> "Ascomycota", "Ascomycota", "Ascomycota", "Ascomycota", "Ascomycota", "Ascomycota", "Ascomycota", "Ascomycota", "Ascomycota",…
#> $ class <chr> "Dothideomycetes", "Eurotiomycetes", "Eurotiomycetes", "Eurotiomycetes", "Eurotiomycetes", "Eurotiomycetes", "Eurotiomycetes"…
#> $ order <chr> "Dothideales", "Eurotiales", "Eurotiales", "Eurotiales", "Eurotiales", "Eurotiales", "Eurotiales", "Eurotiales", "Eurotiales"…
#> $ family <chr> "Dothioraceae", "Aspergillaceae", "Aspergillaceae", "Aspergillaceae", "Aspergillaceae", "Aspergillaceae", "Aspergillaceae", "…
#> $ genus <chr> "Aureobasidium", "Aspergillus", "Penicillium", "Penicillium", "Penicillium", "Penicillium", "Penicillium", "Penicillium", "Pe…
#> $ species <chr> "Aureobasidium Group pullulans", "Aspergillus fumigatus", "Penicillium Group camemberti caseifulvum fuscoglaucum commune", "P…list_family <- split(cheese_taxonomy$asv, cheese_taxonomy$family)
head(list_family, 2)
#> $Aspergillaceae
#> [1] "asv_02" "asv_03" "asv_04" "asv_05" "asv_06" "asv_07" "asv_08" "asv_09"
#>
#> $Debaryomycetaceae
#> [1] "asv_10" "asv_11" "asv_12" "asv_13" "asv_14" "asv_15" "asv_16" "asv_17" "asv_18" "asv_19" "asv_20" "asv_21" "asv_22"The following recipe will
list_family;cheese.rec <-
recipe(cheese ~ ., data = cheese_abundance) %>%
step_aggregate_list(all_numeric_predictors(),
list_agg = list_family, fun_agg = sum) %>%
step_rownormalize_tss(all_numeric_predictors()) %>%
step_select_kruskal(all_numeric_predictors(),
outcome = "cheese", cutoff = 0.05) %>%
prep()
rec
#>
#> ── Recipe ─────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────
#>
#> ── Inputs
#> Number of variables by role
#> outcome: 1
#> predictor: 76
#>
#> ── Training information
#> Training data contained 9 data points and no incomplete rows.
#>
#> ── Operations
#> • Aggregation of: asv_01, asv_02, asv_03, asv_04, asv_05, asv_06, asv_07, asv_08, asv_09, asv_10, asv_11, asv_12, asv_13, ... | Trained
#> • TSS normalization on: Aspergillaceae, Debaryomycetaceae, Dipodascaceae, Dothioraceae, Lichtheimiaceae, Metschnikowiaceae, ... | Trained
#> • Kruskal filtering against cheese on: Aspergillaceae, Debaryomycetaceae, Dipodascaceae, Dothioraceae, Lichtheimiaceae, ... | Trained
juice(rec)
#> # A tibble: 9 × 8
#> sample rind_type cheese Debaryomycetaceae Dipodascaceae Saccharomycetaceae Saccharomycetales fam Incertae sedi…¹ Trichosporonaceae
#> <fct> <fct> <fct> <dbl> <dbl> <dbl> <dbl> <dbl>
#> 1 sample1-1 Natural Saint-Nectaire 0.719 0.0684 0.113 0.00130 0.000702
#> 2 sample1-2 Natural Saint-Nectaire 0.715 0.0725 0.119 0.000801 0.000628
#> 3 sample1-3 Natural Saint-Nectaire 0.547 0.277 0.0938 0.000289 0.00239
#> 4 sample2-1 Washed Livarot 0.153 0.845 0.000854 0 0.000349
#> 5 sample2-2 Washed Livarot 0.150 0.848 0.00106 0 0.000176
#> 6 sample2-3 Washed Livarot 0.160 0.837 0.00108 0 0.000212
#> 7 sample3-1 Washed Epoisses 0.0513 0.944 0.00327 0 0.000140
#> 8 sample3-2 Washed Epoisses 0.0558 0.941 0.00321 0 0.000176
#> 9 sample3-3 Washed Epoisses 0.0547 0.942 0.00329 0 0.000125
#> # ℹ abbreviated name: ¹`Saccharomycetales fam Incertae sedis`To see which variables are kept and the associated p-values, you can use the tidy method on the third step:
tidy(rec, 3)
#> # A tibble: 13 × 4
#> terms pv kept id
#> <chr> <dbl> <lgl> <chr>
#> 1 Aspergillaceae 0.0608 FALSE select_kruskal_WKayj
#> 2 Debaryomycetaceae 0.0273 TRUE select_kruskal_WKayj
#> 3 Dipodascaceae 0.0273 TRUE select_kruskal_WKayj
#> 4 Dothioraceae 0.101 FALSE select_kruskal_WKayj
#> 5 Lichtheimiaceae 0.276 FALSE select_kruskal_WKayj
#> 6 Metschnikowiaceae 0.0509 FALSE select_kruskal_WKayj
#> 7 Mucoraceae 0.0608 FALSE select_kruskal_WKayj
#> 8 Phaffomycetaceae 0.0794 FALSE select_kruskal_WKayj
#> 9 Saccharomycetaceae 0.0273 TRUE select_kruskal_WKayj
#> 10 Saccharomycetales fam Incertae sedis 0.0221 TRUE select_kruskal_WKayj
#> 11 Trichomonascaceae 0.0625 FALSE select_kruskal_WKayj
#> 12 Trichosporonaceae 0.0273 TRUE select_kruskal_WKayj
#> 13 Wickerhamomyceteae 0.177 FALSE select_kruskal_WKayjprotection stack overflow errorIf you have a very large dataset, you may encounter this error:
data("pedcan_expression")
recipe(disease ~ ., data = pedcan_expression) %>%
step_select_cv(all_numeric_predictors(), prop_kept = 0.1)
#> Error: protect(): protection stack overflowIt is linked to how R handles many variables in formulas. To solve it, pass only the dataset to recipe() and manually update roles with update_role(), like in the example below:
recipe(pedcan_expression) %>%
update_role(disease, new_role = "outcome") %>%
update_role(-disease, new_role = "predictor") %>%
step_select_cv(all_numeric_predictors(), prop_kept = 0.1)
#>
#> ── Recipe ─────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────────
#>
#> ── Inputs
#> Number of variables by role
#> outcome: 1
#> predictor: 19196
#>
#> ── Operations
#> • Top CV filtering on: all_numeric_predictors()Like colino, scimo proposes 3 arguments for variable selection steps based on a statistic: n_kept, prop_kept and cutoff.
n_kept and prop_kept deal with how many variables will be kept in the preprocessed dataset, based on an exact count of variables or a proportion relative to the original dataset. They are mutually exclusive.
cutoff removes variables whose statistic is below (or above, depending on the step) it. It could be used alone or in addition to the two others.
scimo doesn’t introduce any additional dependencies compared to recipes.
These binaries (installable software) and packages are in development.
They may not be fully stable and should be used with caution. We make no claims about them.
Health stats visible at Monitor.