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Backwards Incompatible Changes:
locus:
makeChangeoCloneGeneral:
ExampleTrees to use the igprah
1.5.0 format. See https://r.igraph.org/news/index.html#igraph-150 for
details.collapseDuplicates.Diversity:
plotDiversityCurve and
plotAbundanceCurve where limits were not being applied
correctly to zoom in the plots.Gene:
groupGenes where TCR chains where not
being considered when detecting heavy chain sequences prior to
subsetting.General:
ape::read.fastq.General:
junctionAlignment, which counts the number of
nucleotides in the reference germline not present in the alignment, and
the number of V and J nucleotides in the CDR3.Gene Usage:
getFamily where temporary designation
gene names were not being correctly subset to the cluster (family)
level.Lineage:
runPhylip which was causing
buildPhylipLineage to fail when run on Windows.General:
readFastqDb, which reads a repertoire’s .fastq
file and imports the sequencing quality scores for
sequence_alignment. Added
maskPositionsByQuality masks positions that have a
sequencing quality score lower than the specified threshold. The
convenience function getPositionQuality will create a
data.frame with quality scores per position.dplyr dependency to v1.0.padSeqEnds, the argument mod3=TRUE has
been added so that sequences are padded to a length that is a multiple
of 3.translateDNA where NA
values weren’t being translated properly.Amino Acid Analysis:
aminoAcidProperties, which will now default to
nt=TRUE.Diversity: + Added a parameter to countClones
(remove_na) that will remove all rows with NA values in the
clone column if TRUE (default) and issue a warning with how
many were removed. If FALSE, those rows will be kept
instead.
Gene Usage:
getLocus to extract the locus
information from the segment call.getChain to define the chain from
the segment or locus call.countGenes to
give a warning instead of an error so as not to disrupt running
workflows.getSegment where filtering of
non-localized genes was not being applied when called from
getFamily, because the “NL” part of the name was removed
before the filtering step.getAllele,
getGene, getFamily and getLocus,
to parse constant region gene names correctly.getSegment to be able to
parse constant region gene names correctly and not remove the “D” from
“IGHD” when strip_d=TRUE.Lineage:
branch_length argument to
buildPhylipLineage, and augmented graphToPhylo
and phyloToGraph to track intermediate sequence in nodes
for phylo object.countGenes
(remove_na) that will remove all rows with NA values in the
gene column if TRUE (default) and issue a warning with how
many were removed. If FALSE, those rows will be kept
instead.Diversity:
plotDiversityTest that caused all values
of q to appear on the plot rather than just the specified
one.Gene Usage:
groupGenes
where the v_callj_call column for J
gene grouping.groupGenes.only_igh argument of
groupGenes to only_heavy.Backwards Incompatible Changes:
V_CALL (Change-O) as the default to
identify the field that stored the V gene calls, they now use
v_call (AIRR). That means, scripts that relied on default
values (previously, v_call="V_CALL"), will now fail if
calls to the functions are not updated to reflect the correct value for
the data. If data are in the Change-O format, the current default value
v_call="v_call" will fail to identify the column with the V
gene calls as the column v_call doesn’t exist. In this
case, v_call="V_CALL" needs to be specified in the function
call.ExampleDb converted to the AIRR Rearrangement standard
and examples updated accordingly. The legacy Change-O version is
available as ExampleDbChangeo.GRAVY to
gravy);countGenes, countClones (e.g.,
SEQ_COUNT to seq_count)estimateAbundance (e.g., RANK to
rank)groupGenes (e.g., VJ_GROUP to
vj_group)collapseDuplicates and makeChangeoClone
(e.g., SEQUENCE_ID to sequence_id,
COLLAPSE_COUNT to collapse_count)summarizeTrees,
getPathLengths, getMRCA,
tableEdges, testEdges) also return columns in
lower case (e.g., parent, child,
outdegree, steps, annotation,
pvalue)IG_COLOR names converted to official C region
identifiers (IGHA, IGHD, IGHE, IGHG, IGHM, IGHK, IGHL).General:
baseTheme looks is now consistent across
sizing options.cpuCount will now return 1 if the core
count cannot be determined.padSeqEnds wherein the
pad_char argument was being ignored.Diversity:
estimateAbundance slot clone_by now
contains the name of the column with the clonal group identifier, as
specified in the function call. For example, if the function was called
with clone="clone_id", then the clone_by slot
will be clone_id.Lineage:
buildPhylipLineage arguments
vcall, jcall and dnapars_exec to
v_call, j_call and phylip_exec,
respectively.Deprecated:
rarefyDiversity is deprecated in favor of
alphaDiversity, which includes the same functionality.testDiversity is deprecated. The test calculation have
been added to the normal output of alphaDiversity.General:
ape and tibble dependencies.Lineage:
readIgphyml to read in IgPhyML output and
combineIgphyml to combine parameter estimates across
samples.graphToPhylo and phyloToGraph to
allow conversion between graph and phylo formats.Diversity:
estimateAbundance where setting the
clone column to a non-default value produced an error.estimateAbundance through
the min_n, max_n, and uniform
arguments.estimateAbundance. alphaDiversity will call
estimateAbundance for bootstrapping if not provided an
existing AbundanceCurve object.DiversityCurve and
AbundanceCurve objects to accomodate the new diversity
methods.Gene Usage:
groupGenes now supports grouping by V gene, J gene, and
junction length (junc_len) as well, in addition to grouping
by V gene and J gene without junction length. Also added support for
single-cell input data with the addition of new arguments
cell_id, locus, and
only_igh.General:
nonsquareDist function to calculate the
non-square distance matrix of sequences.progressBar, baseTheme,
checkColumns and cpuCount.Diversity:
estimateAbundance, and plotAbundanceCurve,
will now allow group=NULL to be specified to performance
abundance calculations on ungrouped data.Gene Usage:
fill argument to countGenes. When
set TRUE this adds zeroes to the group pairs
that do not exist in the data.groupGenes to group sequences
sharing same V and J gene.Toplogy Analysis:
indirect=TRUE.makeChangeoClone will now issue an error and terminate,
instead of continuing with a warning, when all sequences are not the
same length.General:
IPUAC_AA wherein X was not properly
matching against Q.getAAMatrix to treat * (stop codon)
as a mismatch.General:
readChangeoDb.padSeqEnds function which pads sequences with
Ns to make then equal in length.collapseDuplicates.Diversity:
uniform argument to
rarefyDiversity allowing users to toggle uniform vs
non-uniform sampling.plotAbundance to
plotAbundanceCurve.estimateAbundance return object from a
data.frame to a new AbundanceCurve custom class.plot call for AbundanceCurve
to plotAbundanceCurve.annotate argument from
plotDiversityCurve to plotAbundanceCurve.score argument to
plotDiversityCurve to toggle between plotting diversity or
evenness.plotDiversityTest to generate a
simple plot of DiversityTest object summaries.Gene Usage:
omit_nl argument to getAllele,
getGene and getFamily to allow optional
filtering of non-localized (NL) genes.Lineage:
makeChangeoClone preventing it from
interpreting the id argument correctly.pad_end argument to
makeChangeoClone to allow automatic padding of ends to make
sequences the same length.General:
dry argument to collapseDuplicates
which will annotate duplicate sequences but not remove them when set to
TRUE.collapseDuplicates was returning one
sequence if all sequences were considered ambiguous.Lineage:
makeChangeoClone and buildPhylipLineage for
purposes of (optionally) treating indels as mismatches.buildPhylipLineage when PHYLIP doesn’t
generate inferred sequences and has only one block.General:
readChangeoDb causing the
select argument to do nothing.Gene Usage:
countGenes when the clone argument is
specified to CLONE_COUNT/CLONE_FREQ.General:
readChangeoDb and
writeChangeoDb.General:
seqDist() wherein distance was not
properly calculated in some sequences containing gap characters.getAAMatrix() return
matrix.General:
readChangeoDb() to wrap
data.table::fread() instead of
utils::read.table() if the input file is not
compressed.testSeqEqual(), getSeqDistance()
and getSeqMatrix() to C++ to improve performance of
collapseDuplicates() and other dependent functions.testSeqEqual(), getSeqDistance()
and getSeqMatrix() to seqEqual(),
seqDist() and pairwiseDist(),
respectively.pairwiseEqual() which creates a logical sequence
distance matrix; TRUE if sequences are identical, FALSE if not,
excluding Ns and gaps.X
in translateDNA().collapseDuplicates() wherein the input
data type sanity check would cause the vignette to fail to build under R
3.3.ExampleDb.gz file with a larger, more
clonal, ExampleDb data object.ExampleTrees with a larger set of trees.multiggplot() to gridPlot().Amino Acid Analysis:
normalize=FALSE for charge calculations
to be more consistent with previously published repertoire sequencing
results.Diversity Analysis:
progress argument to
rarefyDiversity() and testDiversity() to
enable the (previously default) progress bar.estimateAbundance() were the function
would fail if there was only a single input sequence per group.data and summary
slots of DiversityTest to uppercase for consistency with
other tools.plot to
plotDiversityCurve for DiversityCurve
objects.Gene Usage:
sortGenes() function to sort V(D)J genes by name
or locus position.clone argument to countGenes() to
allow restriction of gene abundance to one gene per clone.Topology Analysis:
General:
base::nchar().General:
Amino Acid Analysis:
aliphatic() function
were not being passed through the ellipsis argument of
aminoAcidProperties().aminoAcidProperties().AA_TRANS to ABBREV_AA.Diversity:
rarefyDiversity() output.Lineage:
ExampleTrees data with example output from
buildPhylipLineage().General:
getDNADistMatrix() and
getAADistMatrix() to getDNAMatrix and
getAAMatrix(), respectively.getSeqMatrix() which calculates a pairwise
distance matrix for a set of sequences.multiggplot() function for performing multiple
panel plots.Amino Acid Analysis:
gravy(), bulk(),
aliphatic(), polar(), charge(),
countPatterns() and
aminoAcidProperties().Annotation:
getSegment(), getAllele(),
getGene() and getFamily(). May be disabled by
providing the argument strip_d=FALSE.countGenes() to tabulate V(D)J allele, gene and
family usage.Diversity:
countClones(),
estimateAbundance() and plotAbundance().resampleDiversity() to
rarefyDiversity() and changed many of the internals.
Bootstrapping is now performed on an inferred complete relative
abundance distribution.rarefyDiversity() and
testDiversity().rarefyDiversity() and testDiversity() are now
calculated using the mean and standard deviation of the bootstrap
realizations, rather than the median and upper/lower quantiles.plotDiversityCurve().Initial public release.
General:
citation("alakazam")
command.Lineage:
buildPhylipLineage().Lineage:
buildPhylipLineage() would hang on R
3.2 due to R change request PR#15508.Prerelease for review.
These binaries (installable software) and packages are in development.
They may not be fully stable and should be used with caution. We make no claims about them.
Health stats visible at Monitor.