Creating a TI method: Script

definition.yml

The definition has the same hierarchical structure as used by the definition() function. A minimal example

method:
  id: comp_1

parameters:
  - id: component
    default: 1
    type: integer
    distribution:
      type: uniform
      lower: 1
      upper: 10
    description: The nth component to use

wrapper:
  input_required: expression
  input_optional: start_id

You can use our template definition.yml to see all the different possibilities of what can be included in the definition: https://github.com/dynverse/dynmethods/tree/master/template_container . It may also be useful to check out some of the wrappers in dynmethods, e.g.: https://github.com/dynverse/ti_paga/blob/master/definition.yml

Running script

A running script reads in the data, creates a trajectory, and again writes the trajectory to a file. To do the loading and writing tasks, we provide helper libraries for R and python: dyncli and dynclipy.

The wrapper script will typically have the following structure:

• A shebang, such as #!/usr/bin/env Rscript or #!/usr/bin/env python
• A call to dyncli(py) to load in the data, such as dataset <- dyncli::main() or dataset = dynclipy.main()
• All you need to infer the trajectory
• Constructing the trajectory model using any of the wrapping methods
• A call to dyncli(py) to write the data, such as dyncli::write_output(trajectory) or trajectory.write_output()

A minimal example script for R:

#!/usr/bin/env Rscript

dataset <- dyncli::main()

library(dynwrap)
library(dplyr)

# infer trajectory
pca <- prcomp(dataset$expression)

pseudotime <- pca$x[, dataset$parameters$component]

# flip pseudotimes using start_id
if (!is.null(dataset$priors$start_id)) {
  if(mean(pseudotime[start_id]) > 0.5) {
    pseudotime <- 1-pseudotime
  }
}

# build trajectory
trajectory <- wrap_data(cell_ids = rownames(dataset$expression)) %>%
  add_linear_trajectory(pseudotime = pseudotime)

# save output
dyncli::write_output(trajectory, dataset$output)

Make sure this script is executable!

Sys.chmod("run.R", mode = "0770")

and for Python:

#!/usr/bin/env python

import dynclipy
dataset = dynclipy.main()

import pandas as pd
import sklearn.decomposition

# infer trajectory
pca = sklearn.decomposition.PCA()
dimred = pca.fit_transform(dataset['expression'])
pseudotime = pd.Series(
  dimred[:, dataset['parameters']['component']-1],
  index = dataset['expression'].index
)

# build trajectory
trajectory = dynclipy.wrap_data(cell_ids = dataset['expression'].index)
trajectory.add_linear_trajectory(pseudotime = pseudotime)

# save output
trajectory.write_output(dataset['output'])

Testing it out

if (requireNamespace("dyncli", quietly = TRUE)) {
  method <- create_ti_method_definition(
    "definition.yml",
    "run.R"
  )
  dataset <- dynwrap::example_dataset
  trajectory <- infer_trajectory(dataset, method(), verbose = TRUE)
}

# install dynplot to plot the output
if (requireNamespace("dynplot", quietly = TRUE)) {
  dynplot::plot_dimred(trajectory, color_cells = "pseudotime", expression_source = as.matrix(dataset$expression))
}

Interactive development

With the debug parameter, it is possible to enter the script interactively. This makes it easier to develop TI method as you can just load in the input data, and code!

trajectory <- infer_trajectory(dataset, method(), debug = TRUE)

Making your method available for other users

Wrapping a method inside a script does not have any dependency management, and is therefore only meant for development purposes. To deploy your method to other users, check out the containerisation tutorial!

Wrapping a method without dyncli(py)

While using dyncli to load and save the data is the most straightforward when using R and python, it’s also possible to skip these packages and do all the work yourself. Briefly you have to:

• Parse the arguments provided by dynwrap yourself, e.g. --dataset input.h5.
• Read in the hdf5 files generated by dynwrap
• Write the output file in the correct hdf5 format

If after this you’re still convinced you want to do this, please contact us. We’ll be able to provide some further documentation!