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baseq (v0.2.0)

Basic Sequence Processing Tool for Biological Data

Developed by Ambu Vijayan and Dr. J. Sreekumar

baseq is a comprehensive “Molecular Biology Starter Kit” for R. It provides simple, efficient, and well-tested functions for common tasks in sequence processing, analysis, and visualization.

🚀 Quick Reference (Cheat Sheet)

Task	Function	Description
I/O	`read_seq()`	Reads FASTA/FASTQ into DataFrames or Lists.
	`write_seq()`	Writes DataFrames/Lists to FASTA/FASTQ.
Cleaning	`clean_seq()`	Auto-detects DNA/RNA and removes non-bases.
	`filter_fastq_quality()`	Filters large FASTQ files by Phred score.
Central Dogma	`dna_to_protein()`	6-frame translation (optimized).
	`rev_comp()`	Universal reverse complement (DNA/RNA).
	`dna_to_rna()`	Transcription (T -> U).
Discovery	`find_longest_orf()`	Scans all frames for the longest gene.
	`search_motif()`	Finds regex motifs with coordinates.
	`summarize_fasta()`	Returns a “Genomic Report Card” for a file.
Lab Math	`calculate_tm()`	PCR Primer melting temperature.
	`calculate_mw()`	Protein molecular weight (Daltons).
	`simulate_pcr()`	Predicts PCR amplicon size from primers.
Plots	`plot_gc_skew()`	Visualizes G-C distribution.
	`plot_dotplot()`	Visual comparison of two sequences.

New in Version 0.2.0

Performance: Core functions are now vectorized and significantly faster.
Unified API: Smart wrappers like read_seq and clean_seq auto-detect your data.
Discovery: Added ORF finding, Assembly stats, and wet-lab simulators.
Visuals: New publication-quality plots using ggplot2.

Installation

# Install from CRAN (v0.1.4)
install.packages("baseq")

# Install the latest dev version (v0.2.0)
# devtools::install_github("ambuvjyn/baseq")

Getting Started

See the package vignette:

vignette("baseq-vignette", package = "baseq")

These binaries (installable software) and packages are in development.
They may not be fully stable and should be used with caution. We make no claims about them.
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